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Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
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October 14, 2022
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This protocol describes the Capture Hi-C method used to characterize the 3D organization of megabased-sized targeted genomic regions at high-resolution, including boundaries of topologically associating domains (TADs) and long-range chromatin interactions between regulatory and other DNA sequence elements.
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Hauth, A., Galupa, R., Servant, N., Villacorta, L., Hauschulz, K., van Bemmel, J. G., Loda, A., Heard, E. Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C. J. Vis. Exp. (188), e64166, doi:10.3791/64166 (2022).
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