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Hand Dissection of Caenorhabditis elegans Intestines
JoVE Journal
Biologie
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JoVE Journal Biologie
Hand Dissection of Caenorhabditis elegans Intestines

Hand Dissection of Caenorhabditis elegans Intestines

DOI:
10.3791/64120-v

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05:41 min

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September 13, 2022

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, , ,
1Department of Biochemistry & Molecular Biology,Colorado State University

Kapitel

  • 00:05Introduction
  • 00:31Preparation of Stock Solutions and Microcapillary Pipettes
  • 01:10Experimental Preparation
  • 01:56Hand Dissection of the Caenorhabditis elegans Intestine
  • 03:39Results: Isolating Intestines from Adult Caenorhabditis elegans Nematode Worms
  • 05:14Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The present protocol describes a procedure for isolating intestines from adult Caenorhabditis elegans nematode worms by hand for input in genomics, proteomics, microbiome, or other assays.

Tags

Hand DissectionCaenorhabditis ElegansIntestinesPost BiologyMicro Capillary PipettesM9 BathDissection BufferBovine Serum AlbuminLevamisoleNematode Growth MediumHypodermic NeedleChelation Buffer

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