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Investigating Drivers of Antireward in Addiction Behavior with Anatomically Specific Single-Cell Gene Expression Methods
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Investigating Drivers of Antireward in Addiction Behavior with Anatomically Specific Single-Cell Gene Expression Methods
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09:29 min
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August 04, 2022
DOI:
1Daniel Baugh Institute for Functional Genomics and Computational Biology, Department of Pathology, Anatomy, and Cell Biology,Thomas Jefferson University, 2Sidney Kimmel Medical College,Thomas Jefferson University, 3Department of Psychiatry and Behavioral Sciences,Stanford University School of Medicine
Summary
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The combination of laser capture microdissection and microfluidic RT-qPCR provides anatomic and biotechnical specificity in measuring the transcriptome in single neurons and glia. Applying creative methods with a system's biology approach to psychiatric disease may lead to breakthroughs in understanding and treatment such as the neuroinflammation antireward hypothesis in addiction.
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O'Sullivan, S. J., Srivastava, A., Vadigepalli, R., Schwaber, J. S. Investigating Drivers of Antireward in Addiction Behavior with Anatomically Specific Single-Cell Gene Expression Methods. J. Vis. Exp. (186), e64014, doi:10.3791/64014 (2022).
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