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Een geoptimaliseerde single-molecule pull-down test voor kwantificering van eiwitfosforylering
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Biochemie
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JoVE Journal Biochemie
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
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Een geoptimaliseerde single-molecule pull-down test voor kwantificering van eiwitfosforylering

2,553 Views

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07:45 min

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June 06, 2022

DOI:

10.3791/63665-v

DOI:
10.3791/63665-v

•

07:45 min
June 06, 2022

•

7 Views
, , , , , , ,
1Department of Pathology,University of New Mexico School of Medicine, 2Department of Physics and Astronomy,University of New Mexico, 3Comprehensive Cancer Center,University of New Mexico Health Sciences Center

Tags

AI-powered Protein Phosphorylation QuantificationSingle-molecule Pull-down AssaySiMPull ProtocolAntibody LabelingAutofluorescence ReductionSingle-molecule AnalysisPhosphorylation Status Of Individual ProteinsAlternative To Western Blotting And Mass Spectrometry

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Bailey, E. M., Salazar-Cavazos, E., Grattan, R. M., Wester, M. J., Schodt, D. J., Rojo, J. A., Lidke, K. A., Lidke, D. S. An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation. J. Vis. Exp. (184), e63665, doi:10.3791/63665 (2022).

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