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Rapid Genotyping of Mouse Tissue Using Sigma’s Extract-N-Amp Tissue PCR Kit
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Biologie
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JoVE Journal Biologie
Rapid Genotyping of Mouse Tissue Using Sigma’s Extract-N-Amp Tissue PCR Kit

Rapid Genotyping of Mouse Tissue Using Sigma’s Extract-N-Amp Tissue PCR Kit

27,518 Views

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12:39 min

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January 22, 2008

DOI:

10.3791/636-v

DOI:
10.3791/636-v

•

12:39 min
January 22, 2008

•

4 Views
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1Department of Developmental and Cell Biology,University of California, Irvine (UCI)

Summary

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The complete genotyping of a mouse tail sample, including tissue digestion and PCR readout, is done in one and a half hours using Sigma's SYBR Green Extract-N-Amp Tissue PCR kit.

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Rapid GenotypingDNA DetectionPCR AmplificationElectrophoretic GelGenotypePCR-ready DNAGenotyping ProcessMouse Tail SampleDNA ExtractionReal-time PCR AmplificationPositive Sample IdentificationPrompt Genotype Identification

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Cite this Article

Doan, L., Monuki, E. S. Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit. J. Vis. Exp. (11), e636, doi:10.3791/636 (2008).

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