Journal
/
Chemie
/
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
JoVE Journal
Chemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Chemie
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

8,065 Views

•

11:12 min

•

February 07, 2022

DOI:

10.3791/63503-v

DOI:
10.3791/63503-v

•

11:12 min
February 07, 2022

•

35 Views
, , , , ,
1Department of Chemistry,Dalhousie University, 2Proteoform Scientific Inc.

Explore More Videos

Protein PrecipitationOrganic SolventsMass SpectrometrySample PreparationProteome PurificationAcetoneSodium ChlorideMethanolChloroformCentrifugationSDSPelletVortex

Read Article

Cite this Article

Nickerson, J. L., Baghalabadi, V., Dang, Z., Miller, V. A., Little, S. L., Doucette, A. A. Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry. J. Vis. Exp. (180), e63503, doi:10.3791/63503 (2022).

Download .ris file

Copy

Share Video

.

Copy