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Culturas Primárias de Astrócitos de Rato e Microglia e Seu Uso no Estudo da Esclerose Lateral Amiotrófica
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JoVE Journal Neurowissenschaften
Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis

Culturas Primárias de Astrócitos de Rato e Microglia e Seu Uso no Estudo da Esclerose Lateral Amiotrófica

2,966 Views

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09:36 min

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June 23, 2022

DOI:

10.3791/63483-v

DOI:
10.3791/63483-v

•

09:36 min
June 23, 2022

•

17 Views
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1Center for Laser Microscopy, Institute for Physiology and Biochemistry “Jean Giaja”,University of Belgrade Faculty of Biology

Summary

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Apresentamos aqui um protocolo sobre como preparar culturas primárias de células gliais, astrócitos e micróglias de córtices de ratos para imagens de vídeo de lapso de tempo de Ca2+ intracelular para pesquisa sobre fisiopatologia da esclerose lateral amiotrófica no modelo de rato hSOD1G93A .

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Cite this Article

Milićević, K., Korenić, A., Milošević, M., Andjus, P. R. Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis. J. Vis. Exp. (184), e63483, doi:10.3791/63483 (2022).

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