Journal
/
Entwicklungsbiologie
/
In Ovo Intravascular Injection in Chicken Embryos
JoVE Journal
Entwicklungsbiologie
Author Produced
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
In Ovo Intravascular Injection in Chicken Embryos

In Ovo Intravascular Injection in Chicken Embryos

DOI:
10.3791/63458-v

•

07:00 min

•

June 03, 2022

•

, , , , , , , , , , , , ,
1Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China,Yangzhou University, 2Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology,Yangzhou University, 3Animal & Avian Sciences,University of Maryland, College Park, 4College of Biotechnology,Jiangsu University of Science and Technology

Kapitel

  • 00:10Introduction
  • 01:33Fertilized Egg Collection and Preparation
  • 02:39Preparation Before Injection
  • 03:07Windowing
  • 03:36Injection
  • 05:43Representative Results
  • 06:31Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The overall goal of this paper is to describe how to perform in ovo intracellular injection of exogenous materials into chicken embryos. This approach is very useful to study the developmental biology of chicken embryos.

Tags

Chicken EmbryoDevelopmental ResearchIn Ovo InjectionVascular InjectionFertilized EggIncubationNeedle PreparationWindowingPlasmidLentivirusPrimordial Germ CellsTrypan Blue

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development

Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection

Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

Gene Transfer into the Chicken Auditory Organ by In Ovo Micro-electroporation

A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System

A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

In Ovo Electroporation in the Chicken Auditory Brainstem

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development

Read Article