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Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
JoVE Journal
Biochemie
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JoVE Journal Biochemie
Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
DOI:

07:09 min

March 16, 2022

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Kapitel

  • 00:04Introduction
  • 00:59Selection of Labeling Sites
  • 01:29Determination of the R0 Values
  • 03:04Performing FRET Spectral Measurements
  • 04:09Mapping the Distances
  • 04:46Results: Förster Resonance Energy Transfer Mapping of the Preprotein Binding Site
  • 06:30Conclusion

Summary

Automatische Übersetzung

The study details the methodology of FRET mapping including the selection of labeling sites, choice of dyes, acquisition, and data analysis. This methodology is effective at determining binding sites, conformational changes, and dynamic motions in protein systems and is most useful if performed in conjunction with existing 3-D structural information.

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