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cryoSPARC, RELION 및 Scipion을 사용한 견고한 단일 입자 Cryo-Electron Microscopy (cryo-EM) 처리 워크플로우
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Biochemie
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JoVE Journal Biochemie
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
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cryoSPARC, RELION 및 Scipion을 사용한 견고한 단일 입자 Cryo-Electron Microscopy (cryo-EM) 처리 워크플로우

11,013 Views

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13:43 min

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January 31, 2022

DOI:

10.3791/63387-v

DOI:
10.3791/63387-v

•

13:43 min
January 31, 2022

•

76 Views
,
1Institute for Quantitative Biomedicine,Rutgers University, 2Department of Biochemistry & Microbiology,Rutgers University

Summary

Automatically generated

이 문서에서는 3개의 cryo-EM 처리 플랫폼(예: cryoSPARC v3, RELION-3 및 Scipion 3)을 효과적으로 활용하여 고해상도 구조 결정을 위한 다양한 단일 입자 데이터 세트에 적용할 수 있는 단일하고 강력한 워크플로우를 만드는 방법을 설명합니다.

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Cite this Article

DiIorio, M. C., Kulczyk, A. W. A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion. J. Vis. Exp. (179), e63387, doi:10.3791/63387 (2022).

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