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一种重力喂养的经心灌注方法,用于小鼠中枢神经系统的组织学分析
JoVE Journal
Neurowissenschaften
This content is Free Access.
JoVE Journal
Neurowissenschaften
A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System
Please note that all translations are automatically generated.
Click here for the English version.
一种重力喂养的经心灌注方法,用于小鼠中枢神经系统的组织学分析
DOI:
10.3791/63386-v
•
10:37 min
•
January 21, 2022
•
Arnav Rana
,
Paul T. Massa
,
Xin Jie Chen
3
1
Department of Biochemistry and Molecular Biology
,
State University of New York Upstate Medical University
,
2
Department of Neurology
,
State University of New York Upstate Medical University
,
3
Department of Neuroscience and Physiology
,
State University of New York Upstate Medical University
Kapitel
00:04
Introduction
00:46
Construction of Perfusion Apparatus and Dissection Platform
02:40
Preparation of the Paraformaldehyde Solution
04:58
Transcardial “Pump-Free” Perfusion
09:18
Results: Histological Analysis Following Gravity-Fed Perfusion
10:09
Conclusion
Summary
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Automatische Übersetzung
提出了一种用于小鼠中枢神经系统组织学分析的方便的重力喂养灌注方法。磷酸化α突触核蛋白的免疫荧光检测在帕金森病的小鼠模型中得到证明。这项工作还全面描述了经心肌灌注,解剖,组织冷冻/包埋和冷冻切片步骤。
Tags
Gravity-fed Transcardial Perfusion
Histologic Analysis
Mouse Central Nervous System
Fixative Perfusion
Protocol
Apparatus
Buffer Bottles
Micro Spatulas
Tubing
Syringe
PBS
PFA
Styrofoam Block
Article
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