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肌动蛋白动力学、离合器耦合和牵引力分析，促进生长锥体前进

JoVE Journal
Neurowissenschaften

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JoVE Journal Neurowissenschaften

Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance

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肌动蛋白动力学、离合器耦合和牵引力分析，促进生长锥体前进

DOI:

10.3791/63227-v

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07:53 min

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October 21, 2021

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Takunori Minegishi, Ryosuke Fujikawa, Ria Fajarwati Kastian, Yuichi Sakumura, Naoyuki Inagaki

¹Laboratory of Systems Neurobiology and Medicine, Division of Biological Science,Nara Institute of Science and Technology, ²Laboratory of Data-Driven Biology, Division of Biological Science,Nara Institute of Science and Technology

Kapitel

00:04Introduction
00:35Single Speckle Imaging at Neuronal Growth Cones
02:06Traction Force Microscopy at Neuronal Growth Cones
03:46Quantification of Traction Force
06:01Results: Fluorescence Images and Force Mapping of a Neuronal Growth Cone
07:19Conclusion

Summary

Automatische Übersetzung

为了进步，生长锥体必须对外部环境施加牵引力。牵引力的产生取决于肌动蛋白动力学和离合器联轴器。本研究描述了分析肌动蛋白动力学，离合器耦合和生长锥推进牵引力的方法。

Tags

Keywords: Actin Dynamics Clutch Coupling Traction Force Growth Cone Advance Single Speckle Imaging Traction Force Microscopy TMR Ligand Lifeact HaloTag-actin EGFP SDS Gel Substrate

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