Journal
/
Umwelt
/
Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
JoVE Journal
Umwelt
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Umwelt
Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

DOI:
10.3791/63101-v

•

10:03 min

•

December 07, 2021

•

,
1Department of Life Sciences, School of Natural Sciences,Shiv Nadar University

Kapitel

  • 00:04Introduction
  • 00:52Sample Collection, Processing, and Analysis
  • 03:12Degradation of Hydrocarbons
  • 05:04Genomic DNA Isolation of the Pure Culture
  • 06:4916S rRNA Sequencing
  • 08:07Results: Isolation and Characterization of Hydrocarbon Degrading Bacteria from Aquatic Habitats
  • 09:11Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We present the process of isolating, propagating, and characterizing hydrocarbon-degrading bacteria from aquatic habitats. The protocol outlines bacterial isolation, identification by the 16S rRNA method, and testing of their hydrocarbon-degrading potential. This article would help researchers in characterizing microbial biodiversity in environmental samples, and specifically screen for microbes with bioremediation potential.

Tags

Hydrocarbon-degrading MicroorganismsAquatic HabitatsIsolationCultivationIdentificationBacterial SpeciesWater SampleNutrient MediaDilutionColony ScreeningGram StainingGlycerol StocksHydrocarbon Metabolism

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Helminth Collection and Identification from Wildlife

Detection of Foodborne Bacterial Pathogens from Individual Filth Flies

An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates

Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Alternative Method of Removing Otoliths from Sturgeon

Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Characterization of Aquatic Biofilms with Flow Cytometry

Sampling, Sorting, and Characterizing Microplastics in Aquatic Environments with High Suspended Sediment Loads and Large Floating Debris

Read Article