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Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria
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Biochemie
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JoVE Journal Biochemie
Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria

DOI:
10.3791/63055-v

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09:01 min

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January 07, 2022

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, ,
1Muscle Health Research Center, School of Kinesiology and Health Science,York University

Kapitel

  • 00:04Introduction
  • 01:07Functional Isolation of Subsarcolemmal and Intermyofibrillar Mitochondria from Skeletal Muscle
  • 02:15Mitochondrial Protein Import
  • 06:33Results: The Evaluation of Protein Import Capacity into Mitochondria
  • 08:17Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Mitochondria are key metabolic organelles that exhibit a high level of phenotypic plasticity in skeletal muscle. The import of proteins from the cytosol is a critical pathway for organelle biogenesis, essential for the expansion of the reticulum and the maintenance of mitochondrial function. Therefore, protein import serves as a barometer of cellular health.

Tags

MitochondriaProtein ImportSkeletal MuscleBiochemical TechniqueIsolationViabilityIntegrityHomogenizationCentrifugationResuspensionIn Vitro TranslationImport Assay

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