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Una procedura standardizzata per il monitoraggio delle fioriture algali dannose in Cile mediante analisi metabarcodifica
JoVE Journal
Umwelt
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JoVE Journal Umwelt
A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis
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Una procedura standardizzata per il monitoraggio delle fioriture algali dannose in Cile mediante analisi metabarcodifica

4,365 Views

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09:47 min

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August 26, 2021

DOI:

10.3791/62967-v

DOI:
10.3791/62967-v

•

09:47 min
August 26, 2021

•

16 Views
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1Office of Research and Academia-Government-Community Collaboration,Hiroshima University, 2Graduate School of Human and Environmental Studies,Kyoto University, 3Scientific and Biotechnological Bioresource Nucleus,Universidad de La Frontera, 4Centro de Estudios de Algas Nocivas,Instituto de Fomento Pesquero, 5Ciencias del Mar y Recursos Biologicos,Universidad de Antofagasta, 6Departamento de Ciencias Biológicas y Biodiversidad,Universidad de Los Lagos, 7Japan Fisheries Research and Education Agency,Fisheries Resources Institute

Summary

Automatically generated

Questo protocollo introduce fasi di analisi metabarcodifica, mirate ai geni rRNA 16S e rRNA 18S, per monitorare le fioriture algali dannose e il microbioma associato nei campioni di acqua di mare. È un potente strumento basato su molecole, ma richiede diverse procedure, che sono spiegate visivamente qui passo dopo passo.

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Cite this Article

Yarimizu, K., Fujiyoshi, S., Kawai, M., Acuña, J. J., Rilling, J., Campos, M., Vilugrón, J., Cameron, H., Vergara, K., Gajardo, G., Espinoza-González, O., Guzmán, L., Nagai, S., Riquelme, C., Jorquera, M. A., Maruyama, F. A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis. J. Vis. Exp. (174), e62967, doi:10.3791/62967 (2021).

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