Journal
/
Neurowissenschaften
/
Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization
JoVE Journal
Neurowissenschaften
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Neurowissenschaften
Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

DOI:
10.3791/62945-v

•

08:16 min

•

September 20, 2021

•

,
1Center for Hypothalamic Research and Department of Internal Medicine,UTSouthwestern Medical Center at Dallas

Kapitel

  • 00:04Introduction
  • 00:39The Mouse Jugular-nodose Ganglia Sample Collection
  • 02:51Pretreatment of the Slides and In Situ Hybridization (ISH)
  • 04:51Microscopy and Data Analysis
  • 05:34Results: Microscopic Analysis of the Mouse Vagal Afferent Neurons After Multiplex in Situ Hybridization
  • 07:34Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Multiplex in situ hybridization (ISH) was employed to simultaneously visualize the transcripts for two G protein-coupled receptors and one transcription factor in the entire vagal ganglionic complex of the adult mouse. This protocol could be used to generate accurate maps of the transcriptional profiles of vagal afferent neurons.

Tags

Keywords: G Protein-coupled ReceptorVagal Afferent NeuronsMultiplex In Situ HybridizationMouseTranscriptional ProfilingCryosectioningHistological Techniques

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

check_url/de/62945?article_type=v

Double Fluorescence in situ Hybridization in Fresh Brain Sections
check_url/de/62945?article_type=v

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
check_url/de/62945?article_type=v

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
check_url/de/62945?article_type=v

Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)
check_url/de/62945?article_type=v

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
check_url/de/62945?article_type=v

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
check_url/de/62945?article_type=v

In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments
check_url/de/62945?article_type=v

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
check_url/de/62945?article_type=v

Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology
check_url/de/62945?article_type=v

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Read Article