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从成人小鼠腭中分离和培养原代口腔角质形成细胞
JoVE Journal
Biologie
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JoVE Journal Biologie
Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate
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从成人小鼠腭中分离和培养原代口腔角质形成细胞

4,735 Views

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06:28 min

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September 24, 2021

DOI:

10.3791/62820-v

DOI:
10.3791/62820-v

•

06:28 min
September 24, 2021

•

52 Views
, , , , , ,
1Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors,University of Tsukuba, 3International Research Center for Medical Sciences (IRCMS),Kumamoto University, 4Division of Biomimetics, Faculty of Dentistry and Graduate School of Medical and Dental Sciences,Niigata University, 5Faculty of Medicine,University of Tsukuba

Summary

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本方案描述了衍生自成年小鼠上颚的口腔角质形成细胞的分离和培养。还报道了一种使用免疫染色的评估方法。

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Cite this Article

Xuan Ngo, Y., Haga, K., Suzuki, A., Kato, H., Yanagisawa, H., Izumi, K., Sada, A. Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate. J. Vis. Exp. (175), e62820, doi:10.3791/62820 (2021).

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