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Aislamiento y cultivo de queratinocitos orales primarios del paladar del ratón adulto
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September 24, 2021
DOI:
1Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors,University of Tsukuba, 3International Research Center for Medical Sciences (IRCMS),Kumamoto University, 4Division of Biomimetics, Faculty of Dentistry and Graduate School of Medical and Dental Sciences,Niigata University, 5Faculty of Medicine,University of Tsukuba
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El presente protocolo describe el aislamiento y cultivo de queratinocitos orales derivados del paladar del ratón adulto. También se informa de un método de evaluación mediante inmunotinción.
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Xuan Ngo, Y., Haga, K., Suzuki, A., Kato, H., Yanagisawa, H., Izumi, K., Sada, A. Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate. J. Vis. Exp. (175), e62820, doi:10.3791/62820 (2021).
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