Journal
/
Immunologie und Infektion
/
Automated, High-Throughput Detection of Bacterial Adherence to Host Cells
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

DOI:
10.3791/62764-v

•

07:21 min

•

September 17, 2021

•

, , , ,
1Department of Microbial Pathogenesis and Immunology,Texas A&M Health Science Center, 2College of Veterinary Medicine,Texas A&M University

Kapitel

  • 00:04Introduction
  • 00:48Staining
  • 02:20Bacterial Adherence Assay
  • 04:01Automated Fluorescence Imaging, Processing, and Analysis
  • 05:24Results: Analysis of Host Cell-Adhered Bacteria
  • 06:46Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Detection of host-bacterial pathogen interactions based on phenotypic adherence using high-throughput fluorescence labeling imaging along with automated statistical analysis methods enables rapid evaluation of potential bacterial interactions with host cells.

Tags

Bacterial AdherenceHigh-throughput DetectionHost CellsAutomatedTime-savingReproducibleMultiplicity Of InfectionBacterial SuspensionStainingBacterial Adherence AssayA549 CellsPBSFormaldehyde

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

High-throughput Detection Method for Influenza Virus

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

A High-throughput Shigella-specific Bactericidal Assay

Read Article