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Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

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06:12 min

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March 07, 2022

DOI:

10.3791/62730-v

DOI:
10.3791/62730-v

•

06:12 min
March 07, 2022

•

3 Views
, ,
1Graduate Institute of Immunology,National Taiwan University College of Medicine, 2Department of Pathology, Division of Microbiology and Immunology,University of Utah

Summary

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Here we provide a protocol for screening potential transcription factors involved in the development of dendritic cell (DC) using lentiviral transduction of shRNA to obtain stable knockdown cell lines for in vitro DC differentiation.

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Dendritic Cell DevelopmentShort Hairpin RNAGene KnockdownHematopoietic Stem And Progenitor Cell LineIn VitroTranscription FactorsFLT3 LigandBeta EstradiolLentiviral TransductionPuromycinLacZTcf4Id2Flow Cytometry

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Hsiao, Y., Häcker, H., Lee, C. Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro. J. Vis. Exp. (181), e62730, doi:10.3791/62730 (2022).

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