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Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells
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Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells
Kapitel
- 00:05Introduction
- 00:35Flow Cytometry Analysis
- 04:18Data Analysis
- 07:50Results: Analysis of the Restriction Ratio in the Optimal and Suboptimal Data
- 09:15Conclusion
Described here is an established method to determine the extent of HIV-1 restriction by the cellular inhibitory protein SAMHD1. Human myeloid lineage U937 cells are transduced with a SAMHD1 expression vector co-expressing YFP, differentiated and then challenged with HIV-RFP. The level of restriction is determined by flow cytometry analysis.
