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Journal / Immunology and Infection / Confocal Laser Scanning Microscopy-Based…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
 

Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung

Article DOI: 10.3791/62436-v • 15:01 min • September 18th, 2021
September 18th, 2021 •
1, 1, 1, 1, 2, 1,3, 2
1Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 2Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 3Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich

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Summary
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We describe the method for quantitative analysis of the distribution of Aspergillus fumigatus conidia (3 µm in size) in the airways of mice. The method also can be used for the analysis of microparticles and nanoparticle agglomerate distribution in the airways in various pathological condition models.

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Keywords: Confocal Laser Scanning Microscopy Aspergillus Fumigatus Conidia Optical Clearing Mouse Lung Quantitative Analysis Respiratory Tract Mucociliary Clearance Alveolar Macrophages Neutrophils Optical Clearing Benzyl Alcohol Benzyl Benzoate Confocal Microscopy Laser Spectral Range Detector Gain Pinhole Pixel Resolution Z-stack
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