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Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model

Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model

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07:39 min

April 06, 2021

DOI:

07:39 min
April 06, 2021

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Summary

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Synthetic cystic fibrosis sputum medium (SCFM2) can be utilized in combination with both confocal laser scanning microscopy and fluorescence-activated cell sorting to observe bacterial aggregates at high resolution. This paper details methods to assess aggregate populations during antimicrobial treatment as a platform for future studies.

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