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Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells

Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells

3,958 Views

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07:57 min

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April 05, 2021

DOI:

10.3791/62300-v

DOI:
10.3791/62300-v

•

07:57 min
April 05, 2021

•

12 Views
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1Department of Cell and Developmental Biology and the Rocky Mountain Taste and Smell Center,University of Colorado Anschutz Medical Campus

Summary

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The protocol presents a method for culturing and processing lingual organoids derived from taste stem cells isolated from the posterior taste papilla of adult mice.

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Shechtman, L. A., Piarowski, C. M., Scott, J. K., Golden, E. J., Gaillard, D., Barlow, L. A. Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells. J. Vis. Exp. (170), e62300, doi:10.3791/62300 (2021).

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