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Raccolta di dati microscopici di illuminazione criostruzionata da cellule conservate criogenicamente
JoVE Journal
Biologie
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JoVE Journal Biologie
Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
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Raccolta di dati microscopici di illuminazione criostruzionata da cellule conservate criogenicamente

3,805 Views

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11:55 min

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May 28, 2021

DOI:

10.3791/62274-v

DOI:
10.3791/62274-v

•

11:55 min
May 28, 2021

•

4 Views
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1Harwell Science and Innovation Campus,Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology,University of Cambridge, 3ALBA Synchrotron,Beamline 09 – MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry,University of Oxford

Summary

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Questo protocollo dimostra come immaginare campioni biologici crio-conservati utilizzando la microscopia a illuminazione criostrutto. Dimostriamo la metodologia mediante l'imaging del citoscheletro delle cellule U2OS.

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Cite this Article

Vyas, N., Perry, N., Okolo, C. A., Kounatidis, I., Fish, T. M., Nahas, K. L., Jadhav, A., Koronfel, M. A., Groen, J., Pereiro, E., Dobbie, I. M., Harkiolaki, M. Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells. J. Vis. Exp. (171), e62274, doi:10.3791/62274 (2021).

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