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Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung

Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung

6,352 Views

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09:26 min

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January 22, 2021

DOI:

10.3791/62187-v

DOI:
10.3791/62187-v

•

09:26 min
January 22, 2021

•

4 Views
, , , , ,
1School of Life Science,University of Warwick, 2Institute of Microbiology and Infection,University of Birmingham, 3Warwick Antimicrobial Screening Facility, School of Life Science,University of Warwick

Summary

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This workflow can be used to perform antibiotic susceptibility testing using an established ex vivo model of bacterial biofilm in the lungs of individuals with cystic fibrosis. Use of this model could enhance the clinical validity of MBEC (minimal biofilm eradication concentration) assays.

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Cite this Article

Harrington, N. E., Sweeney, E., Alav, I., Allen, F., Moat, J., Harrison, F. Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung. J. Vis. Exp. (167), e62187, doi:10.3791/62187 (2021).

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