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Journal / Neuroscience / Whole-Mount Staining, Visualization, and Analysis …
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Neuroscience

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JoVE Journal Neuroscience
Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds
 

Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds

Article DOI: 10.3791/62126-v • 07:40 min • February 11th, 2021
February 11th, 2021 •
1, 1
1Anatomical Sciences and Neurobiology, University of Louisville

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Summary
Automatic Translation

This paper describes methods for tissue preparation, staining, and analysis of whole fungiform, circumvallate, and palate taste buds that consistently yield whole and intact taste buds (including the nerve fibers that innervate them) and maintain the relationships between structures within taste buds and the surrounding papilla.

Tags

Whole-Mount Staining Visualization Analysis Fungiform Taste Buds Circumvallate Taste Buds Palate Taste Buds Morphology Analysis Transducing Cell Type Numbers Physical Relationships Between Cells Lingual Epithelium Absolute Number Of Cells Volume Of Innervation Technical Variability Reduction Taste Bud Cells Innervation Analysis Potential Circuitry Investigation Disease Processes And Chemotherapies In Taste Function Disrupting Structures And Relationships Single Preparation For All Analyses Avoiding Damage To Epithelium Tissue Freezing And Mold Preparation Thawing And Rinsing Tongue
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