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1John F. Hardesty, MD Department of Ophthalmology and Visual Sciences,Washington University School of Medicine, 2Graduate Program in Neuroscience,Washington University School of Medicine, 3Department of Neuroscience,Washington University School of Medicine, 4Hope Center for Neurological Disorders,Washington University School of Medicine
Kapitel
- 00:05Introduction
- 00:42Imaging Preparation
- 03:44Two-Photon Image Acquisition
- 06:13Mouse Recovery
- 06:46Results: Representative In Vivo Mouse Retina Imaging
- 08:36Conclusion
L'imaging in vivo è un potente strumento per lo studio della biologia in salute e malattia. Questo protocollo descrive l'imaging transpupillare della retina del topo con un microscopio standard a due fotoni. Dimostra anche diversi metodi di imaging in vivoimaging per marcare in modo fluorescente più coorti cellulari della retina.
