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使用纤维素凝块固定 的果蝇 组织用于实时成像
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging
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使用纤维素凝块固定 的果蝇 组织用于实时成像

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08:52 min

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December 22, 2020

DOI:

10.3791/61954-v

DOI:
10.3791/61954-v

•

08:52 min
December 22, 2020

•

3 Views
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1Cell & Developmental Biology, School of Life Sciences,University of Dundee

Summary

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该协议描述了使用纤维蛋白块的样品固定应用，限制漂移，并允许在现场成像过程中添加和冲洗试剂。样品被转移到一滴含有培养基的纤维素中，然后通过添加血栓诱导聚合。

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Cite this Article

Januschke, J., Loyer, N. Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging. J. Vis. Exp. (166), e61954, doi:10.3791/61954 (2020).

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