Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Coculture of Axotomized Rat Retinal Ganglion…
JoVE Journal
Neuroscience

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Neuroscience
Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration
 

Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration

Article DOI: 10.3791/61863-v • 07:57 min • November 2nd, 2020
November 2nd, 2020 •
1,3, 1, 2, 1, 3
1Facultad de CC Experimentales, Universidad Francisco de Vitoria, 2Dept. Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, 3Dept. Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid

Kapitel

Summary
Automatic Translation

We present an in vitro model to assess olfactory ensheathing glia (OEG) neuroregenerative capacity, after neural injury. It is based on a coculture of axotomized adult retinal ganglion neurons (RGN) on OEG monolayers and subsequent study of axonal regeneration, by analyzing RGN axonal and somatodendritic markers.

Tags

Keywords: Axotomized Retinal Ganglion Neurons Olfactory Ensheathing Glia In Vitro Axonal Regeneration Coculture Quantitative Technique Cell Therapy Nervous System Injuries Retinal Dissection Papain DNase Centrifugation Ovo Mucoid Protease Inhibitor NB-B27 Medium PLL-treated Coverslips OEG Monolayer
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter