/
/
Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity
8,307 Views
•
07:58 min
•
August 28, 2020
DOI:
Summary
Automatically generated
This protocol describes the preparation of horizontal hippocampal-entorhinal cortex (HEC) slices from mice exhibiting spontaneous sharp-wave ripple activity. Slices are incubated in a simplified interface holding chamber and recordings are performed under submerged conditions with fast-flowing artificial cerebrospinal fluid to promote tissue oxygenation and the spontaneous emergence of network-level activity.
Read Article
Cite this Article
Whitebirch, A. C. Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity. J. Vis. Exp. (162), e61704, doi:10.3791/61704 (2020).
Copy