Journal
/
Genetik
/
Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
JoVE Journal
Genetik
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Genetik
Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis

DOI:
10.3791/61642-v

•

08:46 min

•

August 26, 2020

•

, , , , , ,
1Department of Hematology,University Medical Centre Ljubljana, 2Clinical Institute for Genomic Medicine,University Medical Centre Ljubljana, 3Faculty of Pharmacy,University of Ljubljana

Kapitel

  • 00:04Introduction
  • 01:02Fluorescence-based Quantitative Real-time PCR (qPCR) and Post-qPCR Analysis by HRM
  • 05:32Agarose Gel Electrophoresis
  • 06:50Results: Detection of the Genetic Variants in the CALR Gene
  • 08:21Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

High resolution melting analysis (HRM) is a sensitive and rapid solution for genetic variant detection. It depends on sequence differences that result in heteroduplexes changing the shape of the melting curve. By combing HRM and agarose gel electrophoresis, different types of genetic variants such as indels can be identified.

Tags

Keywords: Genetic Variant DetectionCALR GeneHigh Resolution Melting AnalysisMyeloproliferative DisordersEssential ThrombocythemiaPrimary MyelofibrosisQPCR HRM Master MixNegative ControlsPositive ControlsNo Template Control (NTC)Optical Adhesive FilmDissociation AnalysisAmplification PlotFluorescence ReadingsPassive Reference

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

check_url/de/61642?article_type=v

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
check_url/de/61642?article_type=v

Detection of Copy Number Alterations Using Single Cell Sequencing
check_url/de/61642?article_type=v

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer’s Disease Gene Susceptibility Assessment
check_url/de/61642?article_type=v

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification
check_url/de/61642?article_type=v

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
check_url/de/61642?article_type=v

High-Resolution Comparison of Bacterial Conjugation Frequencies
check_url/de/61642?article_type=v

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
check_url/de/61642?article_type=v

Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation
check_url/de/61642?article_type=v

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
check_url/de/61642?article_type=v

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Read Article