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Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates
JoVE Journal
Biologie
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JoVE Journal Biologie
Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates

Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates

DOI:
10.3791/61623-v

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10:18 min

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July 09, 2020

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, ,
1Cell Biology and Anatomy, Chicago Medical School,Rosalind Franklin University of Medicine and Science, 2Center for Brain Function and Repair,Rosalind Franklin University of Medicine and Science

Kapitel

  • 00:05Introduction
  • 00:51Aplysia californica Dissection
  • 02:21Voltage-Sensitive Dye (VSD) Staining
  • 03:20Nerve Stimulation Preparation
  • 03:53Suction Electrode Preparation
  • 04:40Imaging Preparation and Optimization
  • 06:04Optical Recording
  • 07:32Results: Representative Photodiode-Based Optical Imaging in Non-Transgenic Invertebrates
  • 09:14Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol presents a method for imaging neuronal population activity with single-cell resolution in non-transgenic invertebrate species using absorbance voltage-sensitive dyes and a photodiode array. This approach enables a rapid workflow, wherein imaging and analysis can be pursued over the course of a single day.

Tags

Keywords: Photodiode ArrayVoltage-sensitive DyesAction PotentialsNeural ActivityOptical ImagingNon-transgenic InvertebratesAplysia CalifornicaCentral Nervous SystemGlutaraldehydeRH 155 DyeMicrodispenserPolyethylene TubingSingle-neuron ResolutionNetwork Dynamics

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