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在血清型 1 型肺炎链球菌 菌株 519/43 中构建突变体
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
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在血清型 1 型肺炎链球菌 菌株 519/43 中构建突变体

1,506 Views

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06:06 min

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September 11, 2020

DOI:

10.3791/61594-v

DOI:
10.3791/61594-v

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06:06 min
September 11, 2020

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2 Views
, , , ,
1Faculty of Infections and Tropical Diseases, Department of Infection Biology,London School of Hygiene and Tropical Medicine, 2Division of Medicine,University College London, 3Division of Infection and Immunity,University College London UCL

Summary

Automatically generated

在这里，我们描述了一种 肺炎链球菌 血清型1菌株519/43，该菌株可以通过利用其自然获取DNA和自杀质粒的能力进行基因改造。作为原理证明，在肺溶血素（ply）基因中制造了同基因突变体。

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Cite this Article

Terra, V. S., Plumptre, C. D., Wall, E. C., Brown, J. S., Wren, B. W. Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43. J. Vis. Exp. (163), e61594, doi:10.3791/61594 (2020).

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