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Analisi dell'ipermutazione somatica nell'introne JH4 delle cellule del Centro Germinale B dalle patch di Mouse Peyer
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer’s Patches
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Analisi dell'ipermutazione somatica nell'introne JH4 delle cellule del Centro Germinale B dalle patch di Mouse Peyer

6,341 Views

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09:35 min

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April 20, 2021

DOI:

10.3791/61551-v

DOI:
10.3791/61551-v

•

09:35 min
April 20, 2021

•

6 Views
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1Department of Biology,The City College of New York and The Graduate Center of The City University of New York, 2Department of Microbiology,Icahn School of Medicine at Mount Sinai

Summary

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Qui viene presentato un saggio per quantificare l'ipermutazione somatica all'interno del locus genico a catena pesante dell'immunoglobulina usando cellule B centro germinali dalle macchie del peyer del topo.

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Somatic HypermutationJH4 IntronGerminal Center B CellsPeyer’s PatchesAntibody DiversificationMolekularbiologiePCRSanger SequencingCell Isolation

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Cite this Article

Sible, E., Zheng, S., Choi, J. E., Vuong, B. Q. Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches. J. Vis. Exp. (170), e61551, doi:10.3791/61551 (2021).

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