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Geração organoide do nervo motor tridimensional
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Three-Dimensional Motor Nerve Organoid Generation

Geração organoide do nervo motor tridimensional

8,203 Views

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09:57 min

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September 24, 2020

DOI:

10.3791/61544-v

DOI:
10.3791/61544-v

•

09:57 min
September 24, 2020

•

61 Views
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1Institute of Industrial Science,The University of Tokyo, 2Department of Chemistry and Biotechnology, School of Engineering,The University of Tokyo, 3Faculty of Science and Engineering,Tecnologico de Monterrey, 4Jiksak Bioengineering, Inc.

Summary

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Este protocolo fornece um procedimento abrangente para fabricar organoide nervoso motor derivado de células iPS humanas através da montagem espontânea de um robusto feixe de axônios estendidos de um esferoide em um chip de cultura tecidual.

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Cite this Article

Osaki, T., Chow, S. Y. A., Nakanishi, Y., Hernández, J., Kawada, J., Fujii, T., Ikeuchi, Y. Three-Dimensional Motor Nerve Organoid Generation. J. Vis. Exp. (163), e61544, doi:10.3791/61544 (2020).

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