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Generazione organoide nervosa motoria tridimensionale
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Three-Dimensional Motor Nerve Organoid Generation
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Generazione organoide nervosa motoria tridimensionale

8,203 Views

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09:57 min

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September 24, 2020

DOI:

10.3791/61544-v

DOI:
10.3791/61544-v

•

09:57 min
September 24, 2020

•

61 Views
, , , , , ,
1Institute of Industrial Science,The University of Tokyo, 2Department of Chemistry and Biotechnology, School of Engineering,The University of Tokyo, 3Faculty of Science and Engineering,Tecnologico de Monterrey, 4Jiksak Bioengineering, Inc.

Summary

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Questo protocollo fornisce una procedura completa per fabbricare organoide del nervo motorio derivato dalle cellule iPS umane attraverso l'assemblaggio spontaneo di un robusto fascio di assoni estesi da uno sferoide in un chip di coltura tissutale.

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Cite this Article

Osaki, T., Chow, S. Y. A., Nakanishi, Y., Hernández, J., Kawada, J., Fujii, T., Ikeuchi, Y. Three-Dimensional Motor Nerve Organoid Generation. J. Vis. Exp. (163), e61544, doi:10.3791/61544 (2020).

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