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1Department of Plant Biology,Carnegie Institute for Science, 2Graduate School of Pharmaceutical Sciences,Kyoto University, 3Department of Biochemistry,Purdue University, 4Department of Chemistry,Purdue University, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences
Kapitel
- 00:04Introduction
- 00:33Immobilized Metal Ion Affinity Chromatography (IMAC) and High pH Reversed-Phase (Hp-RP) Stage Tip Preparation
- 01:10IMAC Stage Tip Phosphopeptide Enrichment
- 02:44Hp-RP C18 Stage Tip Phosphopeptide Fractionation
- 03:37Results: Representative Osmotic Stress Signaling in Arabidopsis
- 05:05Conclusion
Presentert her er en fosfoproteomisk tilnærming, nemlig stoppe og gå ekstraksjon tips basert fosfoproteomisk, som gir høy gjennomstrømning og dyp dekning av Arabidopsis fosfoproteom. Denne tilnærmingen avgrenser oversikten over osmotisk stresssignalering i Arabidopsis.
