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Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons

Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons

DOI:
10.3791/61481-v

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06:54 min

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September 09, 2020

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,
1Department of Neuroscience and Experimental Therapeutics,Texas A&M University Health Science Center, College of Medicine, 2Texas A&M Institute for Neuroscience

Kapitel

  • 00:04Introduction
  • 00:57Infection of Cell Culture at 14 DIV with Adeno-associated Viral (AAV) Vectors
  • 01:43Live Confocal Ca2+ Imaging at 19 21 DIV
  • 05:09Results: Effect of Glutamate Application on Mesencephalic Neurons
  • 06:17Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here we present a protocol to measure in vitro Ca2+ fluxes in midbrain neurons and their downstream effects on caspase-3 using primary mouse midbrain cultures. This model can be employed to study pathophysiologic changes related to abnormal Ca2+ activity in midbrain neurons, and to screen novel therapeutics for anti-apoptotic properties.

Tags

Keywords: Calcium FluxesDopaminergic NeuronsParkinson’s DiseaseNeuroprotective CompoundsPrimary Midbrain NeuronsCalcium-mediated ApoptosisGCaMP6fHEPES Recording BufferGlutamateNBQXConfocal MicroscopyPeristaltic Pump

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