We established the conditions to culture neural progenitor cells from the subventricular zone and dentate gyrus of the adult brain of prairie voles, as a complementary in vitro study, to analyze the sex-dependent differences between neurogenic niches that could be part of functional plastic changes associated with social behaviors.
Ávila-González, D., Young, L. J., Camacho, F., Paredes, R. G., Díaz, N. F., Portillo, W. Culture of Neurospheres Derived from the Neurogenic Niches in Adult Prairie Voles. J. Vis. Exp. (160), e61402, doi:10.3791/61402 (2020).