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Focused Ultrasound Induced Blood-Brain Barrier Opening for Targeting Brain Structures and Evaluating Chemogenetic Neuromodulation
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Focused Ultrasound Induced Blood-Brain Barrier Opening for Targeting Brain Structures and Evaluating Chemogenetic Neuromodulation
Kapitel
- 00:04Introduction
- 00:54Animal Preparation
- 02:30MRI‐Guided Targeting
- 04:35Injection Solution Preparation
- 05:59Insonation
- 07:10Blood Brain Barrier (BBB) Opening Evaluation and Designer Receptors Activated Exclusively by Designer Drugs (DREADD) Stimulation
- 07:47Results: BBB Opening and DREADD Expression Visualization
- 08:11Conclusion
This protocol delineates steps necessary for the gene delivery through focused ultrasound blood brain barrier (BBB) opening, evaluation of the resulting gene expression, and measurement of neuromodulation activity of chemogenetic receptors through histological tests.
