Here, we present a protocol to safely and effectively administer anesthetic gas to mice using a digital, low flow anesthesia system with integrated ventilator and physiological monitoring modules.
Bigiarelli, K., Schepers, L. E., Soepriatna, A. H., FitzMiller, D., Goergen, C. J. Use of an Integrated Low-Flow Anesthetic Vaporizer, Ventilator, and Physiological Monitoring System for Rodents. J. Vis. Exp. (161), e61311, doi:10.3791/61311 (2020).