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Laser-Induced Brain Injury in the Motor Cortex of Rats
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JoVE Journal Neurowissenschaften
Laser-Induced Brain Injury in the Motor Cortex of Rats

Laser-Induced Brain Injury in the Motor Cortex of Rats

DOI:
10.3791/60928-v

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07:05 min

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September 26, 2020

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, , , , , , , , ,
1Division of Anesthesiology and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences,Ben-Gurion University of the Negev, 2Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences,Ben-Gurion University of the Negev, 3Department of Biochemistry and Physiology, Faculty of Biology, Ecology, and Medicine,Oles Honchar Dnipro National University, 4Department of Anesthesiology,Yale University School of Medicine, 5Department of Ophthalmology, Soroka University Medical Center and the Faculty of Health Sciences,Ben-Gurion University of the Negev

Kapitel

  • 00:04Introduction
  • 00:44Laser-Induced Brain Injury
  • 02:03Neurological Severity Score (NSS) Assessment and Post-Injury Manipulations
  • 02:33Brain Infarct Volume Evaluation
  • 03:04Blood Brain Barrier (BBB) Breakage Assessment
  • 04:46Results: Representative Brain Injury Assessment Between Laser, Middle Cerebral Artery (MCAO), and Control Injury Models
  • 06:28Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The protocol presented here shows a technique to create a rodent model of brain injury. The method described here uses laser irradiation and targets motor cortex.

Tags

Laser-induced Brain InjuryMotor CortexSprague Dawley RatsNd:YAG LaserNeurological Severity ScoreTTC StainingEvans Blue DyeTraumatic Brain InjuryBrain Infarct Volume

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