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1The Department of Physiology and Cell Biology, Faculty of Health Sciences,Ben-Gurion University of the Negev, 2The Regenerative Medicine and Stem Cell (RMSC) Research Center,Ben-Gurion University of the Negev, 3The Zlotowski Center for Neuroscience,Ben-Gurion University of the Negev, 4The Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, 5Division of Micro and Nanosystems,KTH Royal Institute of Technology, 6AIMES, Department of Neuroscience,Karolinska Institutet
Kapitel
- 00:05Introduction
- 01:27Seeding the “Brain Side” Channel and Differentiating EZ Spheres into Mixed Neural Cultures
- 03:04Seeding iBMECs (iPSC-Derived Brain Microvascular Endothelial Cells) into the Bottom Channel to Generate the “Blood Side”
- 05:27Initiation of Flow
- 08:12Results: Immunocytochemistry and Permeability of the iPSC-Base Blood-Brain Barrier Chip
- 09:17Conclusion
Kan-beyin bariyeri (BBB) sıkı beyin homeostazı düzenleyen bir çok hücreli nörovasküler birimdir. İnsan iPSC'leri ve çip üzerine organ teknolojilerini birleştirerek, hastalık modellemesi ve CNS ilaç penetrite tahminleri için uygun kişiselleştirilmiş bir BBB çipi ürettik. BBB çipinin üretimi ve işletimi için ayrıntılı bir protokol açıklanmıştır.
