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Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands
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Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands
Kapitel
- 00:05Introduction
- 00:31Metal Limited Neisseria gonorrhoeae Growth
- 01:50Metal Loading
- 02:36Metal-Limited Growth Assay
- 03:50Outer Membrane Metal Transporter Ligand Binding Detection
- 05:38Results: Representative Differential Metal Responsive Protein Production, Growth-Restriction, and Binding
- 06:50Conclusion
We describe here a method for growth of Neisseria gonorrhoeae in metal-restricted liquid medium to facilitate the expression of genes for metal uptake. We also outline downstream experiments to characterize the phenotype of gonococci grown in these conditions. These methods may be adapted to be suitable for characterization of metal-responsive genes in other bacteria.
