/
/
Визуализация и анализ фарингиальных архиерейных артерий с использованием иммуногистохимии и 3D-реконструкции
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Kapitel
- 00:04Introduction
- 00:55Embryo Staining and Embedding
- 03:03Methanol Dehydration and Tissue Clearing
- 04:04Embryo Mounting
- 04:40Third Pharyngeal Arch (PA) Surfacing
- 05:57Surfaced Structure Masking
- 07:01Endothelial Cell Quantification
- 08:19Results: Representative PA and PAA Surfacing and EC Quantification
- 09:28Conclusion
Tags
Keywords:
VisualizationAnalysisPharyngeal Arch ArteriesWhole-mount Immunohistochemistry3D ReconstructionEndothelial Cell QuantificationAortic Arch ArteriesCongenital Heart DiseaseVessel FormationRemodelingLight-sheet MicroscopyMouse EmbryoFixationPermeabilizationBlocking BufferPrimary AntibodySecondary AntibodyPlastic Paraffin Molds
