Journal
/
Biochemie
/
단일 분자 힘 분광학을 위한 OaAEP1 중재된 효소 합성 및 중합단백질의 고정화
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Please note that all translations are automatically generated. Click here for the English version.

단일 분자 힘 분광학을 위한 OaAEP1 중재된 효소 합성 및 중합단백질의 고정화

6,536 Views

•

08:34 min

•

February 05, 2020

DOI:

10.3791/60774-v

DOI:
10.3791/60774-v

•

08:34 min
February 05, 2020

•

1 Views
, , , , , ,
1State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering,Nanjing University

Tags

OaAEP1Enzymatic SynthesisPolymerized ProteinSingle-molecule Force SpectroscopyProtein OligomerPolymerization DegreeProtein-based MaterialCysteinePotassium ChromateSulfuric AcidAPTESSulfo-SMCCGL-ELP-50 Cys-proteinCantilever Surface

Read Article

Cite this Article

Deng, Y., Zheng, B., Liu, Y., Shi, S., Nie, J., Wu, T., Zheng, P. OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy. J. Vis. Exp. (156), e60774, doi:10.3791/60774 (2020).

Download .ris file

Copy

Share Video

.

Copy