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Sintesi enzimatica mediata OaAEP1 e immobilizzazione delle proteine polimerizzate per la spettroscopia a singola molecola

JoVE Journal
Biochemie

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JoVE Journal Biochemie

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

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Sintesi enzimatica mediata OaAEP1 e immobilizzazione delle proteine polimerizzate per la spettroscopia a singola molecola

6,536 Views

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08:34 min

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February 05, 2020

DOI:

10.3791/60774-v

DOI:

10.3791/60774-v

•

08:34 min

February 05, 2020

•

1 Views

Yibing Deng, Bin Zheng, Yutong Liu, Shengchao Shi, Jingyuan Nie, Tao Wu, Peng Zheng

¹State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering,Nanjing University

Tags

OaAEP1 Enzymatic Synthesis Polymerized Protein Single-molecule Force Spectroscopy Protein Oligomer Polymerization Degree Protein-based Material Cysteine Potassium Chromate Sulfuric Acid APTES Sulfo-SMCC GL-ELP-50 Cys-protein Cantilever Surface

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