Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
10,009 Views
•
11:25 min
•
January 25, 2020
DOI:
Summary
Automatically generated
3D multicolor DNA FISH represents a tool to visualize multiple genomic loci within 3D preserved nuclei, unambiguously defining their reciprocal interactions and localization within the nuclear space at a single cell level. Here, a step by step protocol is described for a wide spectrum of human primary cells.
Read Article
Cite this Article
Marasca, F., Cortesi, A., Manganaro, L., Bodega, B. 3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells. J. Vis. Exp. (155), e60712, doi:10.3791/60712 (2020).
Copy