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Ex Vivo Pressurized Hippocampal Capillary-Parenchymal Arteriole Preparation for Functional Study
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JoVE Journal Neurowissenschaften
Ex Vivo Pressurized Hippocampal Capillary-Parenchymal Arteriole Preparation for Functional Study

Ex Vivo Pressurized Hippocampal Capillary-Parenchymal Arteriole Preparation for Functional Study

6,633 Views

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09:15 min

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December 18, 2019

DOI:

10.3791/60676-v

DOI:
10.3791/60676-v

•

09:15 min
December 18, 2019

•

3 Views
, ,
1Department of Anesthesiology,University of Colorado Anschutz Medical Campus, 2Department of Neurological Sciences,University of Vermont Larner College of Medicine, 3Department of Pharmacology,University of Colorado Anschutz Medical Campus

Summary

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The present manuscript details how to isolate hippocampal arterioles and capillaries from the mouse brain and how to pressurize them for pressure myography, immunofluorescence, biochemistry, and molecular studies.

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Rosehart, A. C., Johnson, A. C., Dabertrand, F. Ex Vivo Pressurized Hippocampal Capillary-Parenchymal Arteriole Preparation for Functional Study. J. Vis. Exp. (154), e60676, doi:10.3791/60676 (2019).

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